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A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
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A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Article DOI: 10.3791/57784-v • 06:17 min • August 25th, 2018
August 25th, 2018 •
1, 1,2, 1, 1, 3, 1, 1,2
1Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology, Shandong University, 2Suzhou Institute of Shandong University, 3Department of Urology, Qilu Hospital of Shandong University

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Here we present a protocol to efficiently isolate primary human keratinocytes from adult skin tissues. This method simplifies the conventional procedure by using the ROCK Inhibitor Y-27632 in the inoculation medium to spontaneously separate epidermal cells from dermal cells.

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Primary Human Keratinocytes Isolate Adult Skin Tissue Culture Epidermal Cells Laboratory Applications Clinical Applications Method Wash Tissue Weigh Tissue Rinse Tissue Incubate Tissue Homogenize Tissue Enzyme Mixture Trypsin Dnase I Solution Neutralization Medium Dissociated Cells Cell Strainer
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