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Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes

Article DOI: 10.3791/53174-v • 08:36 min • August 20th, 2015
August 20th, 2015 •
1, 1, 1
1Centre for Eye Research Australia & Department of Ophthalmology, University of Melbourne

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This manuscript provides a step-by-step procedure for the derivation and maintenance of human keratinocytes from plucked hair and subsequent generation of integration-free human induced pluripotent stem cells (hiPSCs) by episomal vectors.

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Integration-free Human Induced Pluripotent Stem Cells HiPSCs Reprogramming Somatic Cells Disease Modeling Pathogenesis In Vitro Drug Screening Gene Therapy Regenerative Medicine Skin Fibroblasts Hematopoietic Cells Epidermal Keratinocytes Non-invasive Method Hair-derived Keratinocytes Plucked Hair Maintenance Of Keratinocytes Episomal Vectors
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