Evaluation of Hemisphere Lateralization with Bilateral Local Field Potential Recording in Secondary Motor Cortex of Mice

Yunan Chen; Ming Li; Ying Zheng; Li Yang

doi:10.3791/59310

Evaluación de la lateralización del hemisferio con registro de potencial de campo local bilateral...

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JoVE Journal Neuroscience

Evaluation of Hemisphere Lateralization with Bilateral Local Field Potential Recording in Secondary Motor Cortex of Mice

Evaluación de la lateralización del hemisferio con registro de potencial de campo local bilateral en corteza motora secundaria de ratones

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07:03 min

July 31, 2019

DOI: 10.3791/59310-v

Yunan Chen^1,2, Ming Li³, Ying Zheng³, Li Yang¹

¹School of Life Sciences,Guangzhou University, ²Institute for Brain Research and Rehabilitation,South China Normal University, ³School of Life Sciences,South China Normal University

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Overview

This study presents in vivo electrophysiological recordings of local field potentials (LFP) in the bilateral secondary motor cortex (M2) of mice, focusing on hemisphere lateralization. The research investigates synchronization differences between APP/PS1 mice and wild-type (WT) controls, revealing altered brain lateralization potentially linked to Alzheimer's disease.

Key Study Components

Area of Science

Neuroscience
Electrophysiology
Alzheimer's Disease Research

Background

Local field potentials (LFPs) are vital for assessing neuronal activity.
Synchronization is important for understanding hemisphere lateralization.
Alzheimer's disease may alter brain lateralization mechanisms.
The study uses a mouse model (APP/PS1) to explore these changes.

Purpose of Study

To evaluate synchronization levels between left and right M2 in APP/PS1 mice.
To investigate the impact of Alzheimer’s pathology on hemisphere lateralization.
To identify potential biomarkers for Alzheimer's treatments through electrophysiological metrics.

Methods Used

In vivo electrophysiological recording of LFPs in the mouse M2 regions.
APP/PS1 mice and wild-type controls were used to assess lateralization effects.
Coherence and cross-correlation analyses were performed on the captured LFP data.
Electrode placement, quality control, and data acquisition were detailed in the protocol.
Methods included high-pass and low-pass filtering and amplification of signals for analysis.

Main Results

Cross-correlation showed that wild-type mice exhibited significant asymmetry in LFPs, unlike APP/PS1 mice, which had increased synchronization.
The findings indicated higher gamma coherence in APP/PS1 mice, suggesting reduced lateralization.
Electrophysiological alterations may connect to the underlying mechanisms of Alzheimer's disease pathology.

Conclusions

The study demonstrates that altered synchronization in M2 could serve as a marker for Alzheimer's pathology.
Insights may contribute to understanding neuronal mechanisms related to hemisphere lateralization and Alzheimer's disease.
This research highlights the importance of electrophysiological measurements in exploring disease models.

Frequently Asked Questions

What advantages does this technique offer?

This technique allows for direct measurement of neuronal activity and synchronization in live animal models, providing crucial insights into brain function and disease.

How is the biological model implemented?

The study uses APP/PS1 transgenic mice to understand the effects of Alzheimer’s disease on brain lateralization and synchrony in M2 regions.

What outcomes are obtained from this protocol?

The protocol yields electrophysiological data that reflect neuronal synchronization, coherence, and potential lateralization changes in the brain.

How can this method be adapted for other studies?

The methods can be applied to different brain regions or diseases, allowing researchers to explore various aspects of neuronal connectivity and activity.

What are the key considerations when conducting these experiments?

It’s crucial to monitor anesthesia depth carefully and to ensure that all surgical and recording procedures are conducted with precision to obtain reliable data.

What limitations should be recognized?

Limitations include the invasiveness of the procedure and potential variability in responses among different mice, which may affect data interpretation.

Presentamos un registro electrofisiológico in vivo del potencial de campo local (LFP) en la corteza motora secundaria bilateral (M2) de ratones, que se puede aplicar para evaluar la lateralización del hemisferio. El estudio reveló niveles alterados de sincronización entre la izquierda y la derecha M2 en ratones APP/PS1 en comparación con los controles WT.

Esta técnica se puede utilizar para revisar algunas propiedades básicas de la electrofisiología interregional para la lateralización del hemisferio, así como la conectividad, la direccionalidad y el acoplamiento. La medición electrofisiológica es un método sensible y eficaz de evaluación en los animales, actividades neuronales. Este protocolo proporciona una mejor manera de apuntalar en la sincronización de señales eléctricas.

La comprensión del mecanismo subyacente de posible lateralización cerebral alterada en la patogénesis de la enfermedad de Alzheimer, puede proporcionar nuevas perspectivas sobre los biomarcadores potenciales para el tratamiento de la enfermedad de Alzheimer. Antes de la cirugía, confirme la profundidad de la anestesia del ratón, realizando un pellizco de cola o dedo del dedo del dedo delfín con fórceps. A continuación, coloque el ratón en el aparato estereotaxico y fije su cabeza.

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