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In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices
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In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices

Article DOI: 10.3791/59534-v • 09:07 min • September 20th, 2019
September 20th, 2019 •
1,2, 1,2, 1,2, 1,2
1CNRS (Integrative Neuroscience and Cognition Center, UMR 8002), 2Université Paris Descartes, Sorbonne Paris Cité

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This protocol describes a set of methods to identify the cell-type specific functional connectivity of long-range inputs from distant brain regions using optogenetic stimulations in ex vivo brain slices.

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In Vivo Intracerebral Stereotaxic Injections Optogenetic Stimulation Long-range Inputs Mouse Brain Slices Functional Connectivity Photostimulation Brain Regions Light Sensitive Ion Channels Surgery Anesthesia Lidocaine Hydrochloride Scalp Cut Stereotaxic Frame Ear Bars Nose Piece Heating Pad Physiological Temperature Skull Cleaning Bregma Lambda Access
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