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Short-Term Free-Floating Slice Cultures from the Adult Human Brain

Article DOI: 10.3791/59845-v • 09:14 min • November 5th, 2019
November 5th, 2019 •
1,2, 1,3, 1, 1, 4, 4, 5, 2, 3, 1
1Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, 2Department of Physiology, Ribeirão Preto Medical School, University of São Paulo, 3Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School, University of São Paulo, 4Department of Anatomy, Institute of Biosciences, São Paulo State University, 5Clinical Hospital at the Ribeirão Preto Medical School, University of São Paulo

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Resumen
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A protocol to prepare free-floating slice cultures from adult human brain is presented. The protocol is a variation of the widely used slice culture method using membrane inserts. It is simple, cost-effective, and recommended for running short-term assays aimed to unravel mechanisms of neurodegeneration behind age-associated brain diseases.

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Slice Cultures Adult Human Brain Brain Tissue Free-floating Protocol Resective Brain Surgery Biochemical Assays Cell Biology Assays Immunohistochemistry Neurodegeneration Brain Diseases Simpler Alternative Cost-effective Alternative Membrane Inserts Meninges Removal Vibratome Specimen Disc Immunohistochemistry Resection Niele Mendes Glaucia Almedia Giovanna Nogueira Salt Bucket Ice Slicing Solution Carbogen Mixture Bubbling Agarose Block Vibratome Section Thickness Vibration Frequency
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