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JoVE Journal Neuroscience
Digital Droplet PCR-metode for kvantifisering av AAV-transduksjonseffektivitet i Murine Retina
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Digital Droplet PCR-metode for kvantifisering av AAV-transduksjonseffektivitet i Murine Retina

Article DOI: 10.3791/63038-v • 09:50 min • December 25th, 2021
December 25th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2
1Molecular Biology, Genetics and Bioengineering Program, Sabanci University, 2Nanotechnology Research and Application Center (SUNUM), Sabanci University

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Denne protokollen presenterer hvordan du kvantifiserer AAV-transduksjonseffektivitet i musenett ved hjelp av digital dråpe PCR (dd-PCR) sammen med liten AAV-produksjon, intravitreal injeksjon, retinal avbildning og retinal genomisk DNA-isolasjon.

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