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Digital Droplet PCR-metod för kvantifiering av AAV-transduktionseffektivitet i Murine Retina

Article DOI: 10.3791/63038-v • 09:50 min • December 25th, 2021
December 25th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2
1Molecular Biology, Genetics and Bioengineering Program, Sabanci University, 2Nanotechnology Research and Application Center (SUNUM), Sabanci University

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Detta protokoll presenterar hur man kvantifieraR AAV-transduktion effektivitet i mus näthinnan med hjälp av digital dropp PCR (dd-PCR) tillsammans med småskaligA AAV produktion, intravitreal injektion, retinal imaging och retinal genomisk DNA isolering.

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Neurovetenskap nummer 178
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