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Lebend-3D-Zell-Immunzytochemie-Assays des pädiatrischen diffusen Mittellinienglioms

Article DOI: 10.3791/63091-v • 09:06 min • November 11th, 2021
November 11th, 2021 •
*1, *1, 2,3, 1
1Department of Onco-hematology, Gene and Cell Therapy, Bambino Gesù Children's Hospital, IRCCS, 2Oncology Service, Children's Health Queensland Hospital and Health Service, 3Child Health Research Centre, The University of Queensland
* These authors contributed equally

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In dieser Studie wird ein Protokoll der Lebend-3D-Zell-Immunzytochemie vorgestellt, das auf eine pädiatrische diffuse Mittellinien-Gliomzelllinie angewendet wird, um die Expression von Proteinen auf der Plasmamembran während dynamischer Prozesse wie 3D-Zellinvasion und -migration in Echtzeit zu untersuchen.

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