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Journal / Biochemistry / 强大的单颗粒冷冻电子显微镜 （cryo-EM） 处理工作流程，具有冷冻 SPARC、RELION…
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Biochemistry

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JoVE Journal Biochemistry
强大的单颗粒冷冻电子显微镜 （cryo-EM） 处理工作流程，具有冷冻 SPARC、RELION 和 Scipion
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强大的单颗粒冷冻电子显微镜 （cryo-EM） 处理工作流程，具有冷冻 SPARC、RELION 和 Scipion

Article DOI: 10.3791/63387-v • 13:43 min • January 31st, 2022
January 31st, 2022 •
1, 1,2
1Institute for Quantitative Biomedicine, Rutgers University, 2Department of Biochemistry & Microbiology, Rutgers University

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本文介绍了如何有效利用三种冷冻电镜处理平台，即 cryoSPARC v3、RELION-3 和 Scipion 3，创建适用于各种单颗粒数据集的单一且强大的工作流程，以实现高分辨率结构测定。

Tags

生物化学，第179期，冷冻电子显微镜，冷冻电镜，单颗粒分析，SPA，冷冻SPARC，RELION，Scipion，图像处理，结构计算，AAV
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