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Biochemistry

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JoVE Journal Biochemistry
En robust arbejdsgang for behandling af kryo-elektronmikroskopi (cryo-EM) med cryoSPARC, RELION og Scipion
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En robust arbejdsgang for behandling af kryo-elektronmikroskopi (cryo-EM) med cryoSPARC, RELION og Scipion

Article DOI: 10.3791/63387-v • 13:43 min • January 31st, 2022
January 31st, 2022 •
1, 1,2
1Institute for Quantitative Biomedicine, Rutgers University, 2Department of Biochemistry & Microbiology, Rutgers University

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Denne artikel beskriver, hvordan man effektivt udnytter tre cryo-EM-behandlingsplatforme, dvs. cryoSPARC v3, RELION-3 og Scipion 3, til at skabe en enkelt og robust arbejdsgang, der gælder for en række enkeltpartikeldatasæt til bestemmelse af struktur i høj opløsning.

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Biokemi Udgave 179 kryo-elektronmikroskopi cryo-EM enkeltpartikelanalyse SPA cryoSPARC RELION Scipion billedbehandling strukturberegning AAV
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