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In Vitro Apical-Out Enteroid Model of Necrotizing Enterocolitis

Article DOI: 10.3791/64003-v • 09:11 min • June 8th, 2022
June 8th, 2022 •
1, 1, 1
1Department of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Oklahoma Health Sciences Center and Center for Pregnancy and Newborn Health

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This protocol describes an apical-out necrotizing enterocolitis (NEC)-in-a-dish model utilizing small intestinal enteroids with reversed polarity, allowing access to the apical surface. We provide an immunofluorescent staining protocol to detect NEC-related epithelial disruption and a method to determine the viability of apical-out enteroids subjected to the NEC-in-a-dish protocol.

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In Vitro Apical-Out Enteroid Model Necrotizing Enterocolitis In Vitro Modeling Potential Therapeutics Oral Administration Apical-out In-a-dish Model Basal Lateral Out Enteroid Cell Media Apical Surface In Vivo Intestinal Inflammation Oral Therapeutics Polarity Reversal Times 3D Enteroids Basolateral Out Enteroids EDTA PBS Basement Membrane Extract Extracellular Matrix Dome P 200 Pipette 15 Milliliter Chronicled Tube
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