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High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
DOI:

08:50 min

May 12, 2023

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Capítulos

  • 00:04Introduction
  • 00:59Apparatus Construction
  • 02:36Flow Cell Fabrication
  • 03:50Assembly of Bead‐Tether Constructs
  • 04:20Identification of Target Constructs
  • 05:00Calibration of Magnetic Force
  • 05:50Bead Tracking for Extension Measurement
  • 06:28Results: Effect of Magnetic Force on DNA Constructs
  • 08:11Conclusion

Summary

Traducción Automática

Here, we describe a high-speed magnetic tweezer setup that performs nanomechanical measurements on force-sensitive biomolecules at the maximum rate of 1.2 kHz. We introduce its application to DNA hairpins and SNARE complexes as model systems, but it will be also applicable to other molecules involved in mechanobiological events.

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