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Basic Methods in Cellular and Molecular Biology
Making Electrocompetent Bacterial Cells
Description
Bacterial transformation can be done by making electrocompetent cells. In this process, foreign DNA enters bacterial cells after an electrical field creates small pores in the cell wall. The cells that can take up DNA from the environment are called competent cells. In the laboratory, scientists can produce electrocompetent ...
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Transcript
La transformation bactérienne est un processus apparaissant naturellement, dans lequel les bactéries ingèrent un ADN étranger et ensuite l'amplifient ou le clonent. Au labo, ce processus peut être artificiellement induit, en utilisant des impulsions de champ électrique haute tension pour créer des pores dans la membrane de la...
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