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Transfection DNA dei mammiferi muscoli scheletrici con In Vivo Elettroporazione

Article DOI: 10.3791/1520-v • 15:56 min • October 19th, 2009
October 19th, 2009 •
1, 1, 1, 1
1Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles

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Si descrivono le modalità dettagliate per la trasfezione efficiente di DNA plasmidico nelle fibre dei muscoli piedi di topi dal vivo utilizzando elettroporazione e la successiva visualizzazione di espressione della proteina usando la microscopia a fluorescenza.

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Biologia Cellulare Numero 32 elettroporazione i muscoli scheletrici plasmidi espressione di proteine mouse microscopia a due fotoni fluorescenza transgenico
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