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Synthesis of an In vivo MRI-detectable Apoptosis Probe

Article DOI: 10.3791/3775-v • 06:58 min • July 31st, 2012
July 31st, 2012 •
1, 2,3, 1, 1
1Division of Cardiovascular Medicine, Department of Medicine, Stanford University Medical Center, 2Division of Cardiology, Department of Medicine, University of California, San Francisco, 3San Francisco VAMC

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Early detection of apoptosis may identify at-risk cell populations in a variety of diseases. Here we demonstrate a method to link an early apoptosis-detection protein (Annexin V) to a MRI-detectable iron oxide nanoparticle (SPIO). This method may be extended to other proteins of interest to generate MRI-detectable molecular imaging probes.

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In Vivo MRI-detectable Apoptosis Probe Cellular Apoptosis Diseases Detect Apoptosis Reversible Stages Pre-clinical Window Preventive Measures Therapeutic Measures Protect The Heart Permanent Damage Annexin V (ANX) Superparamagnetic Iron Oxide (SPIO) Nanoparticles Conjugation Method Oxidation Carboxyl Groups Polysaccharide Shell MRI-detectable Contrast Agent Reducing Buffer Sodium Borohydride Reaction Quenching Microcentrifuge Filtration Dynamic Light Scattering (DLS) Cross-linked Iron Oxide Particle Conjugation Methods Biotin-labeled Nanoparticles
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