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Direkte Imaging af ER Calcium med målrettet-Esterase induceret Dye Loading (TED)
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Direkte Imaging af ER Calcium med målrettet-Esterase induceret Dye Loading (TED)

Article DOI: 10.3791/50317-v • 09:32 min • May 7th, 2013
May 7th, 2013 •
1, 1,2, 1, 1, 3, 1
1Institute for Clinical Neurobiology, University of Wuerzburg, 2Department of Synapses - Circuits - Plasticity, Max Planck Institute of Neurobiology, Martinsried, 3Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians University of Munich

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Målrettet-esterase induceret farvestof belastning (TED) støtter analysen af ​​intracellulære calcium butik dynamik ved fluorescens billeddannelse. Fremgangsmåden baserer på målretning af en rekombinant carboxylesterase til det endoplasmatiske reticulum (ER), hvor det forbedrer den lokale demaskering af syntetisk lav-affinitet Ca

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Cellular Biology neurobiologi Neuroscience molekylærbiologi biokemi Biomedical Engineering Bioengineering Virology medicin anatomi fysiologi kirurgi Endoplasmatisk reticulum ER Calcium Signaling calcium butik calcium imaging calcium indikator metabotropiske signalering Ca Neuroner celler mus dyremodel cellekultur målrettet esterase induceret farvestof lastning billedbehandling
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