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En kvantitativ analys för att studera protein
Flash Freezing and Cryosectioning E12.5 Mouse Brain
Journal (Biology)
Flash Freezing and Cryosectioning E12.5 Mouse Brain
Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
Journal (Biology)
Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
 
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En kvantitativ analys för att studera protein: DNA-interaktioner, Discover transkriptionsregulatorer av genuttryck, och identifiera nya antitumörmedel

Article DOI: 10.3791/50512-v • 06:43 min • August 31st, 2013
August 31st, 2013 •
1, 1, 2, 3, 4, 3,5
1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Program in Molecular Medicine, University of Maryland School of Medicine, 3Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, 4Department of Pharmacology & Experimental Therapeutics, University of Maryland School of Medicine, 5Department of Pathology and Biochemistry & Molecular Biology, University of Maryland School of Medicine

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Vi utvecklade en kvantitativ DNA-bindning ELISA-baserad analys för att mäta transkriptionsfaktor interaktion med DNA. Hög specificitet för Runx2 protein uppnåddes med en konsensus-DNA-erkännande oligonukleotiden och specifik monoklonal antikropp. Kolorimetrisk detektion med en enzym-kopplad antikropp substratreaktionen övervakades i realtid.

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Cellulär Biology transkriptionsfaktorer vitamin D Drug Discovery enzymkopplad immunadsorberande analys (ELISA) DNA-bindande transkriptionsfaktor läkemedelsscreening antikropp
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