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Metabolsk Merking og Membran Fraksjone for komparativ proteomikk analyser av

Article DOI: 10.3791/50535-v • 11:44 min • September 28th, 2013
September 28th, 2013 •
1, 1, 1,2
1Signaling Proteomics, Max Plank Institute of Molecular Plant Physiology, 2Department of Plant Systems Biology, University of Hohenheim

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Her beskriver vi en robust fremgangsmåte for fraksjonering av planteplasmamembraner i detergentresistente og detergentløselige membraner basert på en blanding av umerket og in vivo fullt 15 N merket Arabidopsis thaliana cellekulturer. Fremgangsmåten er brukt for sammenlignende proteomic studier for å forstå signaleringsprosesser.

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Tom verdi Cellular Structures Planter genmodifiserte Arabidopsis Membran Lipids intracellulære signal peptider og proteiner membran proteiner Isotop merking proteomikk planter Arabidopsis thaliana metabolsk merking stabile isotoper merking suspensjon cellekulturer plasma membran fraksjonering to fase system vaskemiddel resistente membraner (DRM) massespektrometri membran microdomains kvantitativ proteomikk
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