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Super-oppløsning Imaging nevrale Dense-core Blemmer

Article DOI: 10.3791/51394-v • 09:30 min • July 2nd, 2014
July 2nd, 2014 •
1,2, 2, 3, 2,4
1Department of Physics, Lewis & Clark College, 2Program in Biochemistry and Molecular Biology, Lewis & Clark College, 3Jungers Center for Neuroscience Research, Oregon Health & Science University, 4Department of Chemistry, Lewis & Clark College

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Vi beskriver hvordan implementere photoactivated lokalisering mikroskopi (PALM)-baserte studier av vesikler i anleggs, dyrkede nerveceller. Viktige deler av vår protokoll inkluderer merking vesikler med photoconvertible chimeras, samle spredte innsamlede råbilder med en super-oppløsning mikros system, og behandlingen de rå bildene for å produsere en super-oppløsning.

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Neuroscience photoactivated lokalisering mikroskopi Dendra2 tett-core vesikkel synapse hippocampus nevron klynge
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