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Combinando singola molecola di manipolazione e di imaging per lo studio delle interazioni proteina-DNA
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Combinando singola molecola di manipolazione e di imaging per lo studio delle interazioni proteina-DNA

Article DOI: 10.3791/51446-v • 14:43 min • August 27th, 2014
August 27th, 2014 •
1,2, 1, 1,3, 1,4,5,6, 1,4
1LENS - European Laboratory for Non-linear Spectroscopy, University of Florence, 2Chemistry Research Laboratory, University of Oxford, 3Department of Biology, University of Florence, 4Department of Physics and Astronomy, University of Florence, 5National Institute of Optics-National Research Council, Italy, 6International Center of Computational Neurophotonics

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Qui si descrive la strumentazione e metodi per la rilevazione di molecole proteiche singolo fluorescenza marcata che interagiscono con una singola molecola di DNA sospeso tra due microsfere otticamente intrappolati.

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Bioingegneria biofisica di singola molecola pinzette ottiche microscopia a fluorescenza legame al DNA delle proteine repressore del lattosio microfluidica
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