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Kombinere Single-molekyl Manipulasjon og bildebehandling for studier av protein-DNA interaksjoner

Article DOI: 10.3791/51446-v • 14:43 min • August 27th, 2014
August 27th, 2014 •
1,2, 1, 1,3, 1,4,5,6, 1,4
1LENS - European Laboratory for Non-linear Spectroscopy, University of Florence, 2Chemistry Research Laboratory, University of Oxford, 3Department of Biology, University of Florence, 4Department of Physics and Astronomy, University of Florence, 5National Institute of Optics-National Research Council, Italy, 6International Center of Computational Neurophotonics

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Her beskriver vi instrumentering og fremgangsmåter for påvisning av enkelt fluorescensmerket-merkede proteinmolekylene vekselvirker med et enkelt DNA-molekyl opphengt mellom to optisk fangede mikrosfærer.

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Bioteknologi enkelt molekyl biofysikk optiske pinsetter fluorescens mikroskopi DNA-bindende proteiner laktose repressor MicroFluidics
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