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Real-time Imaging af Single Engineered RNA-transkripter i levende celler Brug Ratiometrisk bimolekylær Beacons

Article DOI: 10.3791/51544-v • 12:20 min • August 6th, 2014
August 6th, 2014 •
*1, *1, 2, 2, 1
1Department of Bioengineering, University of Pennsylvania, 2Integrated DNA Technologies, Inc.
* These authors contributed equally

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Ratiometrisk bimolekylære beacons (RBMBs) kan anvendes til at afbilde enkelt manipuleret RNA-transkripter i levende celler. Her beskriver vi forberedelse og rensning af RBMBs, levering af RBMBs i celler ved mikroporering og fluorescerende billeddannelse af enkelt RNA-transkripter i real-tid.

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Genetik RNA billedbehandling enkelt molekyle fluorescens levende celle
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