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Developmental Biology

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Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells

Article DOI: 10.3791/53197-v • 10:18 min • October 10th, 2015
October 10th, 2015 •
1,2,3, 4, 1,2,3, 5, 1,2,3, 1,2,3
1Human Genetics and Cognitive Functions, Institut Pasteur, 2CNRS URA 2182 'Genes, synapses and cognition', Institut Pasteur, 3Human Genetics and Cognitive Functions, Université Paris Diderot, Sorbonne Paris Cité, 4Plateforme d' Imagerie Dynamique, Imagopole, CiTech, Institut Pasteur, 5Neuroplasticity and Therapeutics, CECS, I-STEM, AFM, Evry

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Dendritic spines of pyramidal neurons are the sites of most excitatory synapses in mammalian brain cortex. This method describes a 3D quantitative analysis of spine morphologies in human cortical pyramidal glutamatergic neurons derived from induced pluripotent stem cells.

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Three-dimensional Quantification Dendritic Spines Pyramidal Neurons Human Induced Pluripotent Stem Cells Excitatory Synapses Central Nervous System Neuronal Activity Glutamatergic Receptors AMPA Receptors NMDA Receptors Postsynaptic Densities Spine Morphologies Brain Cortex In Vivo Approaches Neuronal Cultures Rodent Tissues Neuropathological Conditions Altered Spine Induction And Maturation Quantitative Analysis Human Cortical Neurons Neural Stem Cells Late Cortical Progenitors Induced Pluripotent Stem Cells Psychiatric Diseases
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