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An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach
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An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach

Article DOI: 10.3791/54808-v • 07:13 min • December 7th, 2016
December 7th, 2016 •
1, 1, 1, 1, 1, 1
1Jules Stein Eye Institute, University of California, Los Angeles

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Subretinal injections are the most common technique for delivering large therapeutic agents such as proteins and viral vectors to photoreceptors and the retinal pigment epithelium. An alternative method in mice that successfully targets the subretinal space with minimal collateral damage and fast recovery times is described here.

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Subretinal Injection Method Transcleral Posterior Approach Viral Vectors Pharmacological Agents Pluripotent Stem Cells Retinal Degenerations Therapeutic Interventions Efficacy Minimal Damage Fast Recovery Sachin P Technician Anesthesia Whisker Trimming Body Temperature Pupil Dilation Methylcellulose Eye Drops Sterilization Fluorescein Preparation Syringe Filling
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