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Metoder for klassifisering av cytoplasmisk foci som mammalstresskorn
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Metoder for klassifisering av cytoplasmisk foci som mammalstresskorn

Article DOI: 10.3791/55656-v • 09:33 min • May 12th, 2017
May 12th, 2017 •
*1,2, *1,2, 1,2,3, 1,2, 1,2, 1,2,4
1Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, 2Department of Medicine, Harvard Medical School, 3Department of Histology and Embryology, Poznan University of Medical Sciences, 4The Broad Institute of Harvard and M.I.T.
* These authors contributed equally

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Stress Granuler (SG) er ikke-membraniske cytoplasmatiske strukturer som dannes i celler utsatt for en rekke spenninger. SG inneholder mRNAer, RNA-bindende proteiner, små ribosomale underenheter, translationsrelaterte faktorer og forskjellige cellesignalproteiner. Denne protokollen beskriver en arbeidsflyt som bruker flere eksperimentelle tilnærminger for å oppdage, karakterisere og kvantifisere bona fide SG-er.

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Cellular Biology RNA granules stress granules mRNA translation repression immunostaining, stressrespons
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