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Eksempel forberedelse og analyse av RNASeq-baserte genuttrykk Data fra sebrafisk

Article DOI: 10.3791/56187-v • 11:42 min • October 27th, 2017
October 27th, 2017 •
*1, *1, 1
1Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine
* These authors contributed equally

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Denne protokollen gir en tilnærming for hele transcriptome analyse fra sebrafisk embryo, larvene, eller sortert celler. Vi inkluderer isolering av RNA, sti analyse av RNASeq data, og qRT-PCR-baserte valideringen av genet uttrykk endringer.

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Genetikk problemet 128 sebrafisk RNAseq transcriptome RNA Danio rerio sti analyse Gene Ontology vilkår flyt sortering fluorescens aktivert celle sortering
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