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Bereiding van de monsters en de analyse van de RNASeq gebaseerde gen expressie gegevens uit de zebravis

Article DOI: 10.3791/56187-v • 11:42 min • October 27th, 2017
October 27th, 2017 •
*1, *1, 1
1Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine
* These authors contributed equally

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Dit protocol biedt een aanpak voor de hele transcriptome analyse van zebravis embryo's, larven, of cellen gesorteerd. Wij omvatten isolatie van RNA, pad analyse van RNASeq gegevens en qRT-PCR-gebaseerde validatie van gen expressie veranderingen.

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Genetica kwestie 128 zebravis RNAseq transcriptoom RNA Danio rerio pad analyse Gene Ontology termen stroom sorteren fluorescentie geactiveerd cel sorteren
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