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Journal / Developmental Biology / 生体内でひと脂肪由来間葉系幹細胞が脂溶性膜の蛍光色素をラット膝関節変形性関節症モデルでの追跡…
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Developmental Biology

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JoVE Journal Developmental Biology
生体内でひと脂肪由来間葉系幹細胞が脂溶性膜の蛍光色素をラット膝関節変形性関節症モデルでの追跡
Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues
Journal (Developmental Biology)
Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues
Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
Journal (Developmental Biology)
Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
 
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生体内でひと脂肪由来間葉系幹細胞が脂溶性膜の蛍光色素をラット膝関節変形性関節症モデルでの追跡

Article DOI: 10.3791/56273-v • 07:31 min • October 8th, 2017
October 8th, 2017 •
*1,2, *1,2, 3, 4, 1,2
1Cellular Biomedicine Group, China, 2Cellular Biomedicine Group, California, 3Department of Orthopedics, Shanghai TCM-Integrated Hospital, 4Department of Orthopedics, Shanghai East Hospital, Tongji University
* These authors contributed equally

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このプロトコルでは、効率的に遠赤色蛍光 (IA) の関節内注入によるラット膝変形性関節症 (KOA) モデルで、セルの永続性とひと脂肪由来間葉系幹細胞 (haMSCs) の体内を監視する方法について説明します。

Tags

発生生物学、問題 128、間葉系茎細胞、体内、関節内注入、蛍光染料のIn vivoイメージング、変形性膝関節内側半月
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