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Forbedret metode til etablering af et In Vitro blod - hjerne barrieren Model baseret på svin hjernen Endothelial Cells

Article DOI: 10.3791/56277-v • 09:23 min • September 24th, 2017
September 24th, 2017 •
1, 1, 2, 2, 1, 2,3, 2, 1
1Lundbeck Foundation Research Initiative on Brain Barriers and Drug Delivery, Department of Biomedicine, Aarhus University, 2Institute of Pharmaceutical Science, King's College London, 3HICoE Centre for Drug Research, Universiti Sains Malaysia

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Formålet med protokollen er at præsentere en optimeret procedure for etableringen af et in vitro- blod - hjerne barrieren (BBB) model baseret på primær svin hjernen endothelial celler (pBECs). Modellen viser høj reproducerbarhed, høj tæthed, og er velegnet til studier af transport og intracellulære menneskehandel drug discovery.

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Medicin sag 127 blod - hjerne barrieren (BBB) centrale nervesystem (CNS) neurovaskulære enhed (NVU) Porcin hjernen endothelial celler (pBECs) transendothelial elektrisk modstand (TEER) mono-kultur (MC) ikke-kontakt Co kultur (NCC) primære isolation intracellulære handel drug discovery neurodegenerative sygdomme
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