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Um baseado em RANKL Osteoclast cultura ensaio de Mouse medula óssea para investigar o papel de mTORC1 na formação de Osteoclast

Article DOI: 10.3791/56468-v • 09:37 min • March 15th, 2018
March 15th, 2018 •
*1, *2, *1, 3, 2, 1, 2, 1
1Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine
* These authors contributed equally

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Este manuscrito descreve um protocolo para isolar e cultura osteoclastos em vitro da medula óssea de rato e estudar o papel do alvo da rapamicina 1 complexo na formação do osteoclast mamíferos/mecanicista.

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Biologia do desenvolvimento edição 133 osteoclasto mTORC1 Raptor osso remodelando biologia celular RANKL M-CSF
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