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Developmental Biology

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JoVE Journal Developmental Biology
माउस अस्थि मज्जा की एक RANKL आधारित अस्थिशोषक संस्कृति परख अस्थिशोषक गठन में mTORC1 की भूमिका की जांच करने के लिए
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माउस अस्थि मज्जा की एक RANKL आधारित अस्थिशोषक संस्कृति परख अस्थिशोषक गठन में mTORC1 की भूमिका की जांच करने के लिए

Article DOI: 10.3791/56468-v • 09:37 min • March 15th, 2018
March 15th, 2018 •
*1, *2, *1, 3, 2, 1, 2, 1
1Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine
* These authors contributed equally

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इस पांडुलिपि को अलग करने और माउस अस्थि मज्जा से इन विट्रो में संस्कृति osteoclasts के लिए एक प्रोटोकॉल का वर्णन है, और अस्थिशोषक गठन में rapamycin परिसर 1 के स्तनधारी/यंत्रवत लक्ष्य की भूमिका का अध्ययन ।

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विकास जीवविज्ञान अंक 133 अस्थिशोषक mTORC1 Raptor अस्थि रिमोल्डिंग सेल बायोलॉजी RANKL एम-सीएसएफ
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