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JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Stive indlejring af faste og plettet, hele, Millimeter-skala prøver for sektion-gratis 3D histologi ved mikro-beregnet tomografi
Solid Lipid Nanoparticles (SLNs) for Intracellular Targeting Applications
Journal (Bioengineering)
Solid Lipid Nanoparticles (SLNs) for Intracellular Targeting Applications
Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers
Journal (Bioengineering)
Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers
 
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Stive indlejring af faste og plettet, hele, Millimeter-skala prøver for sektion-gratis 3D histologi ved mikro-beregnet tomografi

Article DOI: 10.3791/58293-v • 07:41 min • October 17th, 2018
October 17th, 2018 •
1,2, 1,2,3, 1,2, 1,2,3, 1,2, 4, 5, 1,2, 1,2, 1,2
1The Jake Gittlen Laboratories for Cancer Research, Penn State College of Medicine, 2Division of Experimental Pathology, Department of Pathology, Penn State College of Medicine, 3Medical Scientist Training Program, Penn State College of Medicine, 4Center for In Vivo Microscopy, Duke University Medical Center, 5KTM Research

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Vi udviklede protokoller og designet en brugerdefineret apparater at aktivere integrering af millimeter-skala enheder. Vi præsenterer prøve forberedelse procedurer med vægt på integrering i akryl harpiks og polyimid slanger for at opnå stive immobilisering og langtidsopbevaring af prøver for afhøring af væv arkitektur og celle morfologi af mikro-CT.

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Bioteknologi spørgsmål 140 X-ray mikro-beregnet tomografi mikro-CT akryl harpiks indlejring modelorganismer zebrafisk millimeter-skala tredimensional billeddannelse væv arkitektur komplet morfologiske fænotyper phenomics
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