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Immunology and Infection

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JoVE Journal Immunology and Infection
Bestemmelse af regulerende T celle Subsets i Murine Thymus, pancreas afdrypning lymfeknude og milten ved hjælp af flowcytometri
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Bestemmelse af regulerende T celle Subsets i Murine Thymus, pancreas afdrypning lymfeknude og milten ved hjælp af flowcytometri

Article DOI: 10.3791/58848-v • 08:06 min • February 27th, 2019
February 27th, 2019 •
1, 1, 1, 1
1Department of Medical Cell Biology, Uppsala University

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Heri, præsenterer vi en protokol for at forberede enkelt celler fra murine thymus, bugspytkirtlen afløb lymfeknude og milt til yderligere undersøgelse disse celler ved hjælp af flowcytometri. Derudover blev denne protokol brugt til bestemmelse af delmængder af regulerende T-celler ved hjælp af flowcytometri.

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Immunologi og infektion spørgsmålet 144 enkelt celle forberedelse regulerende T-celle flowcytometri Foxp3 Helios Neuropilin 1
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