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JoVE Journal
Immunology and Infection

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Forskelsbehandling af syv immun celle Subsets ved to-fluorokrom Flow flowcytometri
 
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Forskelsbehandling af syv immun celle Subsets ved to-fluorokrom Flow flowcytometri

Article DOI: 10.3791/58955-v 10:58 min March 5th, 2019
March 5th, 2019

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Her præsenterer vi et flow cytometric protokol for at identificere CD4+ og CD8+ T celler, γδ T celler, B-celler, NK-celler og monocytter i humant perifert blod ved hjælp af kun to fluorokromer i stedet for syv. Med denne tilgang, kan fem ekstra markører registreres på de fleste flow cytometers.

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Immunologi og infektion sag 145 flowcytometri immunologi Immunofænotypning humant perifert blod fluorokrom patient multiparametric analyse.
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