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Alzando la Tetra messicano Astyanax mexicanus per analisi di Post-larval fenotipi e Immunohistochemistry intero-monta
Journal JoVE
Génétique
This content is Free Access.
Journal JoVE Génétique
Raising the Mexican Tetra Astyanax mexicanus for Analysis of Post-larval Phenotypes and Whole-mount Immunohistochemistry
DOI:

06:42 min

December 28, 2018

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Chapitres

  • 00:04Titre
  • 00:38Breeding
  • 01:20Hatching Fertilized Eggs
  • 02:16Transfer of Hatched Larvae to Nursery Containers
  • 02:45Preparation of Rotifer-based Fish Food and Feeding of Post-Larval Fish
  • 03:19Whole-Mount Immunohistochemistry of Post-Larval Fish
  • 05:11Results: Surface Fish Produce Greatest Number of Larvae per Spawn
  • 05:56Conclusion

Summary

Traduction automatique

In questo protocollo, dimostriamo come allevare gli adulti Astyanax mexicanus , sollevare le larve ed eseguire immunohistochemistry intero-monta il pesce post-larvale per confrontare i fenotipi della superficie e Grotta morfotipi.

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