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Mimicking and Manipulating Pancreatic Acinar-to-Ductal Metaplasia in 3-dimensional Cell Culture
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Mimicking and Manipulating Pancreatic Acinar-to-Ductal Metaplasia in 3-dimensional Cell Culture

Article DOI: 10.3791/59096-v • 08:16 min • February 11th, 2019
February 11th, 2019 •
1, 1
1Department of Cancer Biology, Mayo Clinic Florida

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Primary acinar cell isolation, protein expression or activity modulation, culture, and down-stream applications are abundantly useful in the ex vivo study of acinar-to-ductal metaplasia (ADM), an early event in the development of pancreatic cancer.

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Pancreatic Acinar-to-ductal Metaplasia 3-dimensional Cell Culture Visualization Ectopically Administered Factors Protein Modulation Real-time Observation Cell Signaling Studies Pancreas Dissection Mouse Euthanization 70% Ethanol Autoclaved Forceps Autoclaved Scissors Incision Fur And Skin Removal Abdominal Cavity View Peritoneal Lining Intestines Displacement Pancreatic Tissue Spleen Separation HBSS Solution
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