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Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
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Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

Article DOI: 10.3791/59551-v • 11:39 min • June 9th, 2019
June 9th, 2019 •
1,2, 3, 3, 3, 1,2, 4, 1,2,4
1Shonai Regional Industry Promotion Center, 2Tsuruoka Metabolomics Laboratory, National Cancer Center, 3Human Metabolome Technologies, Inc, 4Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center

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The purpose of this article is to describe a protocol for extraction of aqueous metabolites from cultured adherent cells for metabolomic analysis, particularly, capillary electrophoresis-mass spectrometry.

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Aqueous Metabolites Cultured Adherent Cells Metabolomic Analysis Capillary Electrophoresis-mass Spectrometry Extraction Protocol Metabolome Analysis Cancer Cells Biomarkers Chemotherapy Response IPS Cells Metabolite Concentrations Culture Preparation HCC827 Cells PC-9 Cells
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