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Genetics

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JoVE Journal Genetics
In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions In Vitro Biochemical As
Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
Journal (Genetics)
Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
Journal (Genetics)
Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
 
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In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions In Vitro Biochemical As

Article DOI: 10.3791/59830-v • 08:14 min • July 17th, 2019
July 17th, 2019 •
*1, *1, *1, *1, 2, 3, 1, 1, 1, 1, 4, 3, 1, 1
1State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 2The Affiliated Hospital of Hangzhou Normal University, 3School of Life Science and Technology, ShanghaiTech University, 4Department of Tissue and Embryology, School of Basic Medical Sciences, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University
* These authors contributed equally

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Présentés ici sont des protocoles pour les essais biochimiques in vitro utilisant des étiquettes de biotine qui peuvent être largement applicables pour étudier les interactions protéines-acides nucléiques.

Tags

Génétique Numéro 149 in vitro analyse biochimique EMSA biotine purification des protéines nucléane hélice, Protéine liant l'ARN acide nucléique
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