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Journal / Genetics / 在人类多能干细胞中使用CRISPR-CAS9生成定义的基因组修饰…
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JoVE Journal Genetics
在人类多能干细胞中使用CRISPR-CAS9生成定义的基因组修饰
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在人类多能干细胞中使用CRISPR-CAS9生成定义的基因组修饰

Article DOI: 10.3791/60085-v • 09:04 min • September 25th, 2019
September 25th, 2019 •
*1, *1, 1,2,3, 1,2,3
1Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, 2Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, 3Department of Pathology and Laboratory Medicine, University of Pennsylvania
* These authors contributed equally

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该协议提供了一种方法，促进在人类多能干细胞中使用CRISPR-CAS9生成定义的异质核苷酸或同源核苷酸变化。

Tags

遗传学， 问题 151， 干细胞， 基因组编辑， CRISPR-CAS9， 单链DNA寡核苷酸， 异体突变， 异源细胞系
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