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Geração de tumores pancreáticos ortotópicos e caracterização ex vivo da citotoxicidade de células T infiltrada tumoral
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Geração de tumores pancreáticos ortotópicos e caracterização ex vivo da citotoxicidade de células T infiltrada tumoral

Article DOI: 10.3791/60622-v • 06:16 min • December 7th, 2019
December 7th, 2019 •
1,2, 1, 2,3
1Division of Cancer, Department of Surgery and Cancer, Imperial College London, 2Centre for Cancer and Inflammation, Barts Cancer Institute, Queen Mary University of London, 3German Center for Neurodegenerative Diseases (DZNE)

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Este protocolo descreve a geração cirúrgica de tumores pancreatic ortotópicos e a digestão rápida de tumores pancreatic recentemente isolados do murine. Após a digestão, populações de células imunes viáveis podem ser usadas para análise a jusante, incluindo a estimulação ex vivo das células T para detecção intracelular de citocina por citometria de fluxo.

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Pesquisa do Câncer Edição 154 Imune digestão tumor célula T ex vivo citocinas pâncreas ortotópico KPC citometria de fluxo intracelular citotoxicidade
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