Journal
/
Biologie
/
Measuring the Confluence of iPSCs Using an Automated Imaging System
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Measuring the Confluence of iPSCs Using an Automated Imaging System

Measuring the Confluence of iPSCs Using an Automated Imaging System

DOI:
10.3791/61225-v

•

11:39 min

•

June 10, 2020

•

, , , , ,
1Genetics and Rare Diseases Research Division,Ospedale Pediatrico Bambino Gesù, IRCCS, 2Department of Science,University Roma Tre, 3Department of Onco-hematology, Gene and Cell Therapy,Ospedale Pediatrico Bambino Gesù, IRCCS

Chapitres

  • 00:04Introduction
  • 00:4296-Well Plate Coating
  • 02:49Induced Pluripotent Stem Cell (iPSC) Culture Maintenance
  • 05:03Cell Confluence Characterization
  • 06:53Cytoskeletal Microfilament Characterization
  • 09:02Results: Representative Analyses of Cell Confluence on Differently Coated Vessels
  • 10:57Conclusion

Summary

Traduction automatique

Traduction automatique

The goal of the protocol is to compare different extracellular matrix (ECM) coating conditions to assess how differential coating affects the growth rate of induced pluripotent stem cells (iPSCs). In particular, we aim to set up conditions to obtain optimal growth of iPSC cultures.

Tags

IPSCsInduced Pluripotent Stem CellsAutomated Imaging SystemConfluenceExtracellular MatrixBasement Membrane MatrixLamininVitronectinFibronectinMouse Embryonic FibroblastsCell CultureCryopreservation

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Bioelectric Analyses of an Osseointegrated Intelligent Implant Design System for Amputees

Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

Visualization of Caenorhabditis elegans Cuticular Structures Using the Lipophilic Vital Dye DiI

A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish

Measuring the Effects of Bacteria on C. Elegans Behavior Using an Egg Retention Assay

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging

A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry

Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder (ALLIGATOR)

Read Article