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Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies

Article DOI: 10.3791/61253-v • 09:35 min • May 27th, 2020
May 27th, 2020 •
1, 1, 1
1Department of Pharmacy, The Faculty of Health and Medical Sciences, University of Copenhagen

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Brain capillary pericytes are essential players in the regulation of blood-brain barrier properties and blood flow. This protocol describes how brain capillary pericytes can be isolated, cultured, characterized with respect to cell type and applied for investigations of intracellular calcium signaling with fluorescent probes.

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Brain Capillary Pericytes Culturing Cytosolic Calcium Measurements Calcium Imaging Studies Intercellular Calcium Signaling Primary Brain Pericytes Cell Culture Calcium Imaging Pericyte Biology Intracellular Signaling Capillaries DMEM Comp T-75 Flask Incubator Light Microscope Confluent PBS Trypsin-EDTA
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