JoVE Journal
Neuroscience
Neuroscience
Un abonnement à JoVE est nécessaire pour afficher ce contenu.
Chapitres
résumé
This is a protocol for acute slice preparation from adult and aging mouse hippocampi that takes advantage of transcardial perfusion and slice cutting with ice-cold NMDG-aCSF to reduce hypoxic damage to the tissue. The resulting slices stay healthy over many hours, and are suitable for long-term patch-clamp and field-recordings.