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Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents

DOI:
10.3791/53008-v

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11:29 min

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September 04, 2015

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, , , , ,
1Department of Physiology, Yong Loo Lin School of Medicine,National University of Singapore, 2Neurobiology/Aging Programme, Life Sciences Institute,National University of Singapore

Chapitres

  • 00:05Titre
  • 01:53Preparation of Artificial Cerebrospinal Fluid (ACSF) and Interface Chamber
  • 02:52Preparation of Acute Hippocampal Slices
  • 06:36Recording of CA3-CA1 Synaptic Responses
  • 08:13Induction Protocols for LTP/LTD
  • 09:25Results: Schematic Representation of a Transverse Hippocampal Slice and Electrode Location for Field-Potential Recording
  • 10:41Conclusion

Summary

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This video article describes experimental procedures to study long-term plasticity and its associative processes such as synaptic tagging, capture and cross-tagging in the CA1 pyramidal neurons using acute hippocampal slices from rodents.

Tags

Synaptic TaggingSynaptic CaptureCross-taggingHippocampusCA1 RegionLTPLTDLate-LTPLong-term PlasticityMemory FormationAcute Hippocampal SlicesField Potential RecordingsRats

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