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A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

Article DOI: 10.3791/61883-v • 06:35 min • December 22nd, 2020
December 22nd, 2020 •
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1Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, 2Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, 3Department of Molecular Biology, Princeton University, 4Molecular Biology Institute, University of California, Los Angeles

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A protocol to co-inject cancer cells and fibroblasts and monitor tumor growth over time is provided. This protocol can be used to understand the molecular basis for the role of fibroblasts as regulators of tumor growth.

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Mouse Model Cancer-associated Fibroblasts Tumor Growth Molecular Basis Functional Role Melanoma Therapies DMEM FBS Conical Tubes Frozen Cells Sterile Conditions Centrifuge Cell Pellet Tissue Culture Plate Serum Incubator
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