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Journal / Genetics / 沙飞（木薯）胚胎微注射为CRISPR/Cas9穆塔赫内西…
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Genetics

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JoVE Journal Genetics
沙飞（木薯）胚胎微注射为CRISPR/Cas9穆塔赫内西
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沙飞（木薯）胚胎微注射为CRISPR/Cas9穆塔赫内西

Article DOI: 10.3791/61924-v • 05:44 min • November 17th, 2020
November 17th, 2020 •
1, 1, 1, 1, 2, 2
1Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2University of Maryland Insect Transformation Facility, The Institute for Bioscience and Biotechnology Research

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该协议详细介绍了CRISPR/Cas9在沙蝇中靶向诱变的步骤：胚胎采集、注射、昆虫饲养和识别，以及兴趣突变的选择。

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本月在JoVE，第165期，昆虫，基因组编辑，胚胎注射
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