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Biochemistry

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JoVE Journal Biochemistry
Isolierung von aktivem Caenorhabditis elegans Kernextrakt und Rekonstitution für in vitro Transkription
Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
Journal (Biochemistry)
Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach
Journal (Biochemistry)
Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach
 
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Isolierung von aktivem Caenorhabditis elegans Kernextrakt und Rekonstitution für in vitro Transkription

Article DOI: 10.3791/62723-v • 06:09 min • August 11th, 2021
August 11th, 2021 •
1, 1
1Department of Translational Medicine and Physiology, Elson S. Floyd College of Medicine, Washington State University

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Hier beschreiben wir ein detailliertes Protokoll zur Isolierung von aktivem Kernextrakt aus Larvenstadium 4 C. elegans und visualisierung der Transkriptionsaktivität in einem in vitro System.

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Biochemistry C. elegans Kernextrakt In-vitro-Transkription Balch-Homogenisator nicht-radioaktiver Nachweis
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