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Immunology and Infection

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JoVE Journal Immunology and Infection
Generación de transgénicos y knockouts en especies de Strongyloides por microinyección
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Journal (Immunology and Infection)
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
Journal (Immunology and Infection)
Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
 
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Generación de transgénicos y knockouts en especies de Strongyloides por microinyección

Article DOI: 10.3791/63023-v • 09:42 min • October 7th, 2021
October 7th, 2021 •
1, 1,2
1Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 2Molecular Biology Institute, University of California, Los Angeles

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El kit de herramientas genómicas funcionales para los nematodos parásitos Strongyloides stercoralis y Strongyloides ratti incluye transgénesis, mutagénesis mediada por CRISPR / Cas9 y ARNi. Este protocolo demostrará cómo utilizar la microinyección intragonadal para introducir transgenes y componentes CRISPR en S. stercoralis y S. ratti.

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Inmunología e Infección Número 176
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