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Conteggio rapido delle unità formanti colonie in formato piastra a 96 pozzetti applicato al microbioma Drosophila
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Conteggio rapido delle unità formanti colonie in formato piastra a 96 pozzetti applicato al microbioma Drosophila

Article DOI: 10.3791/64298-v • 12:55 min • January 13th, 2023
January 13th, 2023 •
1, 1,2
1Department of Embryology, Carnegie Institution for Science, 2Department of Biology, Johns Hopkins University

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Questo metodo quantifica l'abbondanza microbica utilizzando un formato di piastra a 96 pozzetti per le unità formanti colonie di placca (CFU) e viene applicato al microbioma di Drosophila in campioni di omogenato di mosca interi. I CFU vengono conteggiati con un software di analisi delle immagini automatizzato fornito qui.

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Biologia Numero 191
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