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Basic Methods in Cellular and Molecular Biology
Plasmid DNA Cleanup from Bacterial Cells
Description
Plasmid DNA cleanup from bacterial cells is a lab method for separating plasmid DNA from genomic DNA, proteins, ribosomes, and the bacterial cell wall. A plasmid is a small, circular, double-stranded DNA molecule that carries a specific DNA fragment. After transformation into a host organism, the plasmid is replicated and ma...
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Transcript
I plasmidi sono molecole circolari, extra cromosomiche, di DNA e in biologia molecolare fungono da vettori, o vettori, per uno specifico frammento di DNA. I batteri vengono utilizzati per replicare i plasmidi, in modo che il DNA di interesse sia prodotto in serie. Il processo attraverso il quale i ricercatori ottengono plasmi...
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